Sunday, June 10, 2012

June 2012 -- older kids, pawpaws, and monkeyflowers


Blog update Sunday, June 10, 2012

It’s amazing! Tonight CH was asleep by 7:45pm, TM was asleep before then (or pretending well enough that it was true soon after), and now it’s just me and the cat on the sofa! Q is travelling again, but he’s away for just a short trip this time.

CH is almost 14mo old now! In the last two weeks she’s really grown up. It’s quite obvious now that she’s definitely a toddler, not just a 1-yr old. Seeing her grow up is exciting and sad at the same time. TM is looking forward to being in 1st grade next year, which is good. We’re working on her math, but I keep forgetting to keep quizzing her, and without constant practice, she slides backwards rather quickly. Her reading is much better, which is fun. Q keeps threatening to go to business school, which is something he’s always wanted to do. I’ll be interested (very) to see what he finally decides…

Work is going well – or at least it’s fun, which is close enough to “going well”. There are so many things I wish we could do with my projects that it’s easy to get sidetracked and disheartened, but I have to remember that “it is what it is”, and we’ll do what we can with what we have. Thanks for the reminder, Dad J. Meanwhile, it’s fun!

Last week was a pawpaw week – I collected some leaf tissue for DNA extraction and basic genotyping, looked at some results from my last experiment, and messed around in the greenhouse some more. I absolutely love going to the greenhouse! Pawpaw plants are wonderful. The leaves are big (huge!), they have a delicate green smell, and the new shoot tips droop strongly downward, then straighten out as they get older. It’s such an interesting plant! They’re in the Annonaceae, family, along with the custard apple, which was one of my favorite fruits in India. Asimina triloba is the northernmost genus of the family (I think I’m safe saying that), which explains its oddly tropical appearance.

Last week I also went to the large commercial nursery with whom we are collaborating on the pawpaw grant. What an amazing setup they have! They can propagate hundreds of thousands of plants in a week or two. It’s absolutely astounding. And of course the smell is wonderful – green growing things abound. They also use mostly biological controls to keep infestations at bay. I hope I get to learn more about that as time goes by, because it’s fascinating to hear about pathogenic insects and their predators.

My favorite part of the trip was an infrared temperature gun – you point it at something, squeeze the trigger, and it tells you the temperature of whatever you’re pointing it at! My hand was 31C, the pots and the floor were at 25C, my purse was a little lower… oh so much fun! I want one.

Last week was pawpaw week, this coming week is Mimulus week. I’ll be collecting tissue for RNA extraction! I hope I get it done well and quickly. There are over 200 plants, and I won’t be collecting from all of them, but from as many as I can (the plants with fully expanded but not dying leaves). Once we get all the RNA extracted, we’ll ship samples off to be sequenced! I’m excited and a bit nervous about that – data from sequencing this sort of thing can be around a terabyte. How in the world does one deal with that much data? I’ll find out.

I’m so lucky to be working on these projects. They’re fun and interesting and take up whatever extra energy I have left over from mom-life. Speaking of mom-life, why in the world is it okay to pay someone ELSE to take care of your kids, but if you take care of your own kids, you’re unemployed (with all the associated stigma)? How is that logical? Huh? Not that I want to leave my job, no, I’ve just been pondering the logistics of raising kids.

Saturday, April 7, 2012

Pawpaw, Mimulus, cats and kids

It's been forever, so I'll send out a small update. Someday I should start putting photos here, but that would mean actually taking photos and posting them. For some reason, since soon after our new baby (CH) started walking at 10mo, my free time has substantially dwindled. One of my good friends happens to have enough time to come watch her for a few hours a day, thank goodness, giving me time to get some work done.

Work is great! Well, other than the fact that I had to replant my Mimulus guttatus project because the growth chamber settings were off. That was a bad day. But now they're off to the races again and this time I have high hopes that we'll get nice big healthy leaves for some RNA-sequencing.

And the pawpaw project is moving along -- they've ordered some plants, and I've written protocols for inducing polyploidy in shoots and seedlings. Now all we have to do is give it a try! I'm excited to see what happens.

TM just woke up -- it's nice having a 6-yr old around to help with CH. And by the way she finally learned to ride her bike! Thank you, Pedalheads. It's marvelous.

I've been making fondant roses, a few at a time, for an Easter cake. They're easy and SO beautiful! It's nice to be able to make something pretty that doesn't take very long. Of course, when I say it doesn't take very long, that doesn't mean I have time to make 2 dozen cookies with roses on them...no, this is just for Easter.

And Yay Easter! I just love Easter.

And now the craziness starts again -- kids, kids, cleaning, kids, trying to fit in work time... yup. But I love my life.